Canada is one of the signatories on the Convention on Biodiversity and its Aichi Biodiversity Targets. With these ambitious conservation targets, there is a need to better understand the distribution of threats to natural systems across the Canadian landscape. Global footprint maps have shown Canada as relatively ‘wild’ however, the threats mapped represent only a subset of threats relevant to Canada as the country still lacks a national scale cumulative threat study. Mapping threats to biodiversity on a national scale will help determine what systems are most intact and which areas are heavily threatened.